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| Registros recuperados: 32 | |
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| Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle. |
| Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the... |
| Tipo: Text |
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf |
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| Shibata, Marília; Coelho, Cileide Maria Medeiros; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Campos, José Marcello Salabert de; Maraschin, Marcelo. |
| This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.00.00-0 Botânica; 2.03.03.00-9 Fisiologia Vegetal; 5.02.01.04-2 Sementes Florestais maturation; Recalcitrant seed; Light microscopy; Transmission electron microscopy; Flow cytometry Botânica. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43381 |
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| Hoyos,Julián Mauricio Ágredo; Alves,Eduardo; Rozwalka,Luciane Cristina; Souza,Elaine Aparecida de; Zeviani,Walmes Marques. |
| Pseudocercospora griseola, the etiologic agent of angular leaf spot of common bean (Phaseolus vulgaris), is an important disease in all bean-producing regions worldwide and may cause extremely high yield losses. The control of this disease is made more difficult by the pathogen's genetic variability and the inefficiency of fungicides. In this study, of 26 essential oils tested at different concentrations, 25 demonstrated efficiency in affecting the germination of strains 63-31 and 63-63 of the pathogen, reaching inhibition levels of between 80% and 100%. Cymbopogon citratus and Cymbopogon martinii inhibited conidia germination at all concentrations; Eugenia caryophyllata, Cinnamomum sp., Thymus vulgaris, Matricaria recutita, Cordia verbenacea, Origanum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Angular leaf spot; Transmission electron microscopy; Alternative control of plant disease. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000300002 |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig. |
| Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011 |
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| Catroxo,M. H. B; Miranda,L.B; Lavorenti,A; Petrella,S; Melo,N. A; Martins,A. M. C. P. R. F. |
| Coronaviruses are enveloped, positive-stranded RNA, belongs to the Nidovirales order, Coronaviridae family and are classified into three groups. Groups 3a and 3c comprising the avian coronaviruses. They are worldwide in distribution and can cause respiratory, enteric and in some cases hepatitis and neurological diseases in a wide variety of animals, resulting in economic losses to breeders and breeding. In this study, we describe an outbreak of diarrhea in a herd of capybaras found in the city of Piracicaba, São Paulo, Brazil. The animals had sudden symptoms of anorexia, profuse and watery diarrhea, yellowish or greenish, dehydration, weight loss, sharp and twisting of limbs leading to death in less than a week, mostly weaned and with an age of three... |
| Tipo: Journal article |
Palavras-chave: Coronavirus; Capybaras; Transmission electron microscopy. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200035 |
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| Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José. |
| Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant. F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400 |
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| Pereira,Ricardo Borges; Lucas,Gilvaine Ciavareli; Perina,Fabiano José; Alves,Eduardo. |
| Rust is considered the most important disease in coffee because it causes severe defoliation in plants and, consequently, reduction in productivity. This study evaluated the in vitro effect of essential oils of cinnamon, citronella, lemongrass, clove, tea tree, thyme, neem and eucalyptus on the germination of urediniospores of Hemileia vastatrix; the effectiveness of these oils to control rust on seedlings of coffee cultivars Catucaí 2SL, Catuaí IAC 62 and Mundo Novo 379/19 in the greenhouse; and the effect of more promising oils on urediniospores of H. vastatrix by transmission electron microscopy (TEM). All the essential oils inhibited the germination of urediniospores with increasing concentrations. All oils promoted partial control of the disease in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hemileia vastatrix; Alternative control; Transmission electron microscopy. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000100002 |
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| Catroxo,M. H. B; Pongiluppi,T; Melo,N. A; Milanelo,L; Petrella,S; Martins,A. M. C. P. F; Rebouças,M. M. |
| Avianpox is a highly contagious disease infecting both commercial and wild birds, causing great damages to breeders and breeding. Caused by DNA viruses of the family Poxviridae, genus Avipoxvirus, if manifest through 3 forms, cutaneous, diphtheric and septicemic. In June 2003, during illegal commercialization of Brazilian birds, 800 wild birds (Paroaria dominicana, Sporophila caerulescens and Sporophila albogularis) were apprehended and being forwarded to the CRAS (Wild Animals Recovery Center), Tietê Ecological Park. After one month, birds presented cutaneous lesions in the beak and feet and anorexia, emaciation, locomotion difficulties, diarrhea, dehydration and death. Among the 800 birds, 500 died and 40 these (15 Paroaria dominicana, 15 Sporophila... |
| Tipo: Journal article |
Palavras-chave: Poxvirus; Paroaria dominicana; Sporophila caerulescens; Sporophila albogularis; Transmission electron microscopy. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200043 |
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| PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C.. |
| ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545 |
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| Ribeiro, Luciene Oliveira; Universidade Federal de Lavras; Paiva, Luciano Vilela; Universidade Federal de Lavras; Pádua, Marlúcia Souza; Universidade Federal de Lavras; Santos, Breno Régis; Universidade Federal de Alfenas; Alves, Eduardo; Universidade Federal de Lavras; Stein, Vanessa Cristina; Universidade Federal de Goiás. |
| This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were... |
| Tipo: Laboratory analysis |
Palavras-chave: 5.01.00.00-9 Agronomia 5.01.00.00-9 scanning electron microscopy; Transmission electron microscopy; Musa sp.; Cytology; Embryogenesis Agronomia. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14501 |
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| Revajová, Viera; Loószová, Adriana; Goldová, Maria; Zibrín, Martin; Herich, Robert; Levkut, Mikulas. |
| In order to morphological study of non-specific host immune response, cross-species experimental infection of Leghorn chicks with partridge specific coccidium, Eimeria procera (106 oocyst/per birds) was done. Schizonts of E. procera were histologically observed in all the parts of small intestine and caeca of chicks. Heterophilic and lymphocytic infiltration in lamina propria, and damage of villi intestine were observed. Schizonts were studied by electron microscope after 12, 30, and 60 hours post-infection (h pi). Results demonstrated immature and structurally damaged schizonts at 12 h pi, delayed maturation of first generation schizonts at 30 h pi, and phagocytosis of parasites at 60 h pi by intraepithelial lymphocytes. |
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Palavras-chave: Eimeria procera; Transmission electron microscopy; Specific and non-specific host; Intraepithelial lymphocytes. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/144 |
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| SOUZA,Leonor Cristina Silva; HANADA,Rogerio Eiji; ASSIS,Luiz Alberto Guimarães; CAMELO-GARCÍA,Viviana M.; REZENDE,Jorge Alberto Marques; YUKI,Valdir A.; KITAJIMA,Elliot W.. |
| ABSTRACT The habanero chilli pepper, Capsicum chinense is an important crop in the Amazon Basin, mainly grown by small-scale producers. Capsicum chinense plants in an experimental field in the northern Brazilian state of Amazonas were found exhibiting characteristic symptoms of viral infection. Leaf sap from symptomatic plants examined under a transmission electron microscope revealed the presence of elongated flexuous particles and isometric particles. Using molecular assays, the viruses were identified as pepper yellow mosaic virus (PepYMV) and cucumber mosaic virus (CMV). Aphids, identified as Aphis gossypii, were found colonizing the C. chinense plants in the field and may be the vector for both PepYMV and CMV. We report the first occurrence of these... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Cucumovirus; Potyvirus; Transmission electron microscopy; RT-PCR; Aphis gossypii. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100005 |
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| Registros recuperados: 32 | |
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